Like most websites The Translational Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Disease Area Cancer, Personalized medicine, Public health

Improving Absorption Measurements through Light Source Selection


Stray light limits the maximum absorbance level achievable in a spectral measurement. Once the stray light limit is reached, sample dilution or a shorter pathlength is required to measure more concentrated samples. In this Application Note, we show the impact of stray light on absorbance levels and describe how optimizing the choice of light source can minimize stray light and increase the maximum absorbance measured.


The maximum absorbance level achievable with a spectrometer is limited, in part, by stray light. Stray light is undesired light of any wavelength that reaches the detector, including light that reaches the detector from an unintended source (reflection and scatter from optical bench components). Since the detector cannot distinguish stray light from light coming from the intended light path, the system’s dynamic range is lessened and the maximum achievable absorbance measurement level is reduced.

Read the full article now

Log in or register to read this article in full and gain access to The Translational Scientist’s entire content archive. It’s FREE!


Subscribe to The Translational Scientist Newsletters

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].

Register to The Translational Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts