Achieve Rapid TSA-Based Multiplexing with LabSat® Research
contributed by Epredia |
Unlike some of the existing multiplex approaches, such as nextgeneration sequencing, mPCR, or mass spectrometry, multiplex immunofluorescence – also referred to as fluorescent multiplex immunohistochemistry – provides an edge to understand the co-expression and spatial distribution of multiple targets without compromising the tissue integrity.
Tyramide Signal Amplification (TSA) is an immunostaining method designed for its high sensitivity, capable of detecting low expressed epitopes that generates precise and clear results. Tyramide is coupled to a fluorophore to generate a signal amplification. Via a reaction mediated by HRP (horseradish peroxidase) coupled with the secondary antibody (AbII), tyramide turns into its active conformation and covalently binds to tyrosine residues proximal to the target epitope.
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